No detection of the NS5B S282T mutation in treatment-naïve genotype 1 HCV/HIV-1 coinfected patients using deep sequencing

J Clin Virol. 2013 Dec;58(4):726-9. doi: 10.1016/j.jcv.2013.09.022. Epub 2013 Oct 3.

Abstract

Background: The S282T mutation is the main variant described associated with resistance to nucleos(t)ide analogues hepatitis C virus (HCV) NS5B polymerase inhibitors.

Objective: We aimed here to investigate whether this substitution pre-existed in treatment naive HCV/HIV-1 coinfected patients.

Study design: NS5B polymerase deep sequencing was performed at a median coverage per base of 4471 in 16 patient samples.

Results: No S282T variant was detected in the 16 analyzed samples.

Conclusion: This finding is in agreement with the high genetic barrier of nucleoside analogues NS5B polymerase inhibitors and the clinical efficacy of these compounds.

Keywords: Deep sequencing; HCV; NS5B; Resistance; S282T.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antiviral Agents / pharmacology
  • Base Sequence
  • Cohort Studies
  • Coinfection
  • Drug Resistance, Viral / genetics
  • Female
  • Genotype
  • HIV Infections / virology*
  • Hepacivirus / enzymology
  • Hepacivirus / genetics*
  • Hepatitis C / virology*
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Male
  • Molecular Sequence Data
  • Mutation*
  • RNA, Viral / blood
  • Sequence Alignment
  • Sequence Analysis, RNA
  • Sequence Homology, Amino Acid
  • Viral Nonstructural Proteins / antagonists & inhibitors
  • Viral Nonstructural Proteins / genetics*

Substances

  • Antiviral Agents
  • RNA, Viral
  • Viral Nonstructural Proteins
  • NS-5 protein, hepatitis C virus