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Cell Stem Cell. 2014 Jan 2;14(1):107-20. doi: 10.1016/j.stem.2013.09.014. Epub 2013 Oct 17.

The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak.

Author information

1
Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
2
Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
3
Gene Therapy Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
4
Bioinformatics Group, Children's Medical Research Institute, Westmead NSW 2145, Australia.
5
Cancer Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia.
6
Gene Therapy Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
7
Cancer Research Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia.
8
Embryology Unit, Children's Medical Research Institute, Westmead NSW 2145, Australia; Sydney Medical School, University of Sydney, NSW 2006, Australia. Electronic address: ptam@cmri.org.au.

Abstract

Mouse epiblast stem cells (EpiSCs) can be derived from a wide range of developmental stages. To characterize and compare EpiSCs with different origins, we derived a series of EpiSC lines from pregastrula stage to late-bud-stage mouse embryos. We found that the transcriptomes of these cells are hierarchically distinct from those of the embryonic stem cells, induced pluripotent stem cells (iPSCs), and epiblast/ectoderm. The EpiSCs display globally similar gene expression profiles irrespective of the original developmental stage of the source tissue. They are developmentally similar to the ectoderm of the late-gastrula-stage embryo and behave like anterior primitive streak cells when differentiated in vitro and in vivo. The EpiSC lines that we derived can also be categorized based on a correlation between gene expression signature and predisposition to differentiate into particular germ-layer derivatives. Our findings therefore highlight distinct identifying characteristics of EpiSCs and provide a foundation for further examination of EpiSC properties and potential.

PMID:
24139757
DOI:
10.1016/j.stem.2013.09.014
[Indexed for MEDLINE]
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