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Nucleic Acids Res. 2014 Jan;42(2):1224-44. doi: 10.1093/nar/gkt894. Epub 2013 Oct 16.

The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana.

Author information

1
Department of Gene Expression, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan, Poland, Department of Molecular and Cellular Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Poznan, Poland, Max Planck Institute for Plant Breading Research, 50829, Germany, Biomathematics and Statistics Scotland (BioSS), James Hutton Institute, Dundee DD2 5DA, Scotland, UK, Cell and Molecular Sciences, James Hutton Institute, Dundee DD2 5DA, Scotland, UK and Division of Plant Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, Scotland, UK.

Abstract

How alternative splicing (AS) is regulated in plants has not yet been elucidated. Previously, we have shown that the nuclear cap-binding protein complex (AtCBC) is involved in AS in Arabidopsis thaliana. Here we show that both subunits of AtCBC (AtCBP20 and AtCBP80) interact with SERRATE (AtSE), a protein involved in the microRNA biogenesis pathway. Moreover, using a high-resolution reverse transcriptase-polymerase chain reaction AS system we have found that AtSE influences AS in a similar way to the cap-binding complex (CBC), preferentially affecting selection of 5' splice site of first introns. The AtSE protein acts in cooperation with AtCBC: many changes observed in the mutant lacking the correct SERRATE activity were common to those observed in the cbp mutants. Interestingly, significant changes in AS of some genes were also observed in other mutants of plant microRNA biogenesis pathway, hyl1-2 and dcl1-7, but a majority of them did not correspond to the changes observed in the se-1 mutant. Thus, the role of SERRATE in AS regulation is distinct from that of HYL1 and DCL1, and is similar to the regulation of AS in which CBC is involved.

PMID:
24137006
PMCID:
PMC3902902
DOI:
10.1093/nar/gkt894
[Indexed for MEDLINE]
Free PMC Article
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