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Science. 2013 Oct 18;342(6156):357-60. doi: 10.1126/science.1241459.

Genomically recoded organisms expand biological functions.

Author information

1
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA.

Abstract

We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655 with synonymous UAA codons, which permitted the deletion of release factor 1 and reassignment of UAG translation function. This GRO exhibited improved properties for incorporation of nonstandard amino acids that expand the chemical diversity of proteins in vivo. The GRO also exhibited increased resistance to T7 bacteriophage, demonstrating that new genetic codes could enable increased viral resistance.

PMID:
24136966
PMCID:
PMC4924538
DOI:
10.1126/science.1241459
[Indexed for MEDLINE]
Free PMC Article

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