Format

Send to

Choose Destination
Dev Cell. 2013 Oct 14;27(1):5-18. doi: 10.1016/j.devcel.2013.09.003.

WT1 maintains adrenal-gonadal primordium identity and marks a population of AGP-like progenitors within the adrenal gland.

Author information

1
Institute of Biology Valrose, iBV, University of Nice Sophia-Antipolis, 06108 Nice Cedex 2, France; INSERM UMR 1091, CNRS UMR 7277 Parc Valrose, 06108 Nice Cedex 2, France.

Abstract

Adrenal glands and gonads share a common primordium (AGP), but the molecular events driving differentiation are poorly understood. Here we demonstrate that the Wilms tumor suppressor WT1 is a key factor defining AGP identity by inhibiting the steroidogenic differentiation process. Indeed, ectopic expression of WT1 precludes differentiation into adrenocortical steroidogenic cells by locking them into a progenitor state. Chromatin immunoprecipitation experiments identify Tcf21 and Gli1 as direct targets of WT1. Moreover, cell lineage tracing analyses identify a long-living progenitor population within the adrenal gland, characterized by the expression of WT1, GATA4, GLI1, and TCF21, that can generate steroidogenic cells in vivo. Strikingly, gonadectomy dramatically activates these WT1(+) cells and leads to their differentiation into gonadal steroidogenic tissue. Thus, our data describe a mechanism of response to organ loss by recreating hormone-producing cells at a heterotopic site.

PMID:
24135228
PMCID:
PMC4032791
DOI:
10.1016/j.devcel.2013.09.003
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center