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J Clin Microbiol. 2014 Jan;52(1):302-6. doi: 10.1128/JCM.01656-13. Epub 2013 Oct 16.

Evaluation of a multitarget real-time PCR assay for detection of Bordetella species during a pertussis outbreak in New Hampshire in 2011.

Author information

1
New Hampshire Department of Health and Human Services, Division of Public Health Services, Concord, New Hampshire, USA.

Abstract

A multitarget real-time PCR assay with three targets, including insertion sequence 481 (IS481), IS1001, and an IS1001-like element, as well as pertussis toxin subunit S1 (ptxS1), for the detection of Bordetella species was evaluated during a pertussis outbreak. The sensitivity and specificity were 77 and 88% (PCR) and 66 and 100% (culture), respectively. All patients with an IS481 C(T) of <30 also tested positive by ptxS1 assay and were clinical pertussis cases. No patients with IS481 C(T) values of ≥40 tested positive by culture. Therefore, we recommend that culture be performed only for specimens with IS481 C(T) values of 30 ≤ CT <40.

PMID:
24131698
PMCID:
PMC3911459
DOI:
10.1128/JCM.01656-13
[Indexed for MEDLINE]
Free PMC Article

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