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J Clin Microbiol. 2014 Jan;52(1):298-301. doi: 10.1128/JCM.02274-13. Epub 2013 Oct 16.

Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent Plasmodium vivax infections in Cambodia.

Author information

1
Division of Infectious Diseases, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.

Abstract

A heteroduplex tracking assay used to genotype Plasmodium vivax merozoite surface protein 1 was adapted to a capillary electrophoresis format, obviating the need for radiolabeled probes and allowing its use in settings where malaria is endemic. This new assay achieved good allelic discrimination and detected high multiplicities of infection in 63 P. vivax infections in Cambodia. More than half of the recurrent parasitemias sampled displayed identical or highly related genotypes compared to the initial genotype, suggesting that they represented relapses.

PMID:
24131693
PMCID:
PMC3911425
DOI:
10.1128/JCM.02274-13
[Indexed for MEDLINE]
Free PMC Article

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