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Biochem Biophys Res Commun. 2013 Nov 8;441(1):139-43. doi: 10.1016/j.bbrc.2013.10.025. Epub 2013 Oct 12.

miRNA-205 affects infiltration and metastasis of breast cancer.

Author information

1
Department of Chest Surgery, The First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, China; Department of Tumor, SenGong Hospital of Shaanxi, Xi'an 710300, China.

Abstract

BACKGROUND:

An increasing number of studies have shown that miRNAs are commonly deregulated in human malignancies, but little is known about the function of miRNA-205 (miR-205) in human breast cancer. The present study investigated the influence of miR-205 on breast cancer malignancy.

METHODS:

The expression level of miR-205 in the MCF7 breast cancer cell line was determined by quantitative (q)RT-PCR. We then analyzed the expression of miR-205 in breast cancer and paired non-tumor tissues. Finally, the roles of miR-205 in regulating tumor proliferation, apoptosis, migration, and target gene expression were studied by MTT assay, flow cytometry, qRT-PCR, Western blotting and luciferase assay.

RESULTS:

miR-205 was downregulated in breast cancer cells or tissues compared with normal breast cell lines or non-tumor tissues. Overexpression of miR-205 reduced the growth and colony-formation capacity of MCF7 cells by inducing apoptosis. Overexpression of miR-205 inhibited MCF7 cell migration and invasiveness. By bioinformation analysis, miR-205 was predicted to bind to the 3' untranslated regions of human epidermal growth factor receptor (HER)3 mRNA, and upregulation of miR-205 reduced HER3 protein expression.

CONCLUSION:

miR-205 is a tumor suppressor in human breast cancer by post-transcriptional inhibition of HER3 expression.

KEYWORDS:

Breast cancer; Tumor suppressor; miRNA-205; miRNAs

PMID:
24129185
DOI:
10.1016/j.bbrc.2013.10.025
[Indexed for MEDLINE]

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