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Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18501-6. doi: 10.1073/pnas.1311193110. Epub 2013 Oct 14.

Cytosolic reducing power preserves glutamate in retina.

Author information

1
Departments of Biochemistry, Pharmacology, Chemistry, and Ophthalmology, University of Washington, Seattle, WA 98195.

Abstract

Glutamate in neurons is an important excitatory neurotransmitter, but it also is a key metabolite. We investigated how glutamate in a neural tissue is protected from catabolism. Flux analysis using (13)C-labeled fuels revealed that retinas use activities of the malate aspartate shuttle to protect >98% of their glutamate from oxidation in mitochondria. Isolation of glutamate from the oxidative pathway relies on cytosolic NADH/NAD(+), which is influenced by extracellular glucose, lactate, and pyruvate.

PMID:
24127593
PMCID:
PMC3831988
DOI:
10.1073/pnas.1311193110
[Indexed for MEDLINE]
Free PMC Article

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