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Nano Lett. 2013;13(11):5608-14. doi: 10.1021/nl403252x. Epub 2013 Oct 21.

Nanoscale ligand spacing influences receptor triggering in T cells and NK cells.

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1
Department of Materials and ‡Division of Cell and Molecular Biology, Imperial College London , Exhibition Road, London SW7 2AZ, United Kingdom.

Abstract

Bioactive nanoscale arrays were constructed to ligate activating cell surface receptors on T cells (the CD3 component of the TCR complex) and natural killer (NK) cells (CD16). These arrays are formed from biofunctionalized gold nanospheres with controlled interparticle spacing in the range 25-104 nm. Responses to these nanoarrays were assessed using the extent of membrane-localized phosphotyrosine in T cells stimulated with CD3-binding nanoarrays and the size of cell contact area for NK cells stimulated with CD16-binding nanoarrays. In both cases, the strength of response decreased with increasing spacing, falling to background levels by 69 nm in the T cell/anti-CD3 system and 104 nm for the NK cell/anti-CD16 system. These results demonstrate that immune receptor triggering can be influenced by the nanoscale spatial organization of receptor/ligand interactions.

PMID:
24125583
PMCID:
PMC4288448
DOI:
10.1021/nl403252x
[Indexed for MEDLINE]
Free PMC Article
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