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Am J Clin Pathol. 2013 Nov;140(5):658-69. doi: 10.1309/AJCPDFBEBQZW1OI7.

CD177 expression on neutrophils: in search of a clonal assay for myeloid neoplasia by flow cytometry.

Author information

1
Dept of Pathology, Room 528A RB&C, University Hospitals Case Medical Center, 11100 Euclid Ave, Cleveland, OH 44106; e-mail: hjm2@case.edu.

Abstract

OBJECTIVES:

To determine whether the fraction of CD177+ neutrophils might be altered in clonal myeloid disorders, similar to the skewed κ/λ ratio for B-cell lymphomas, and could be used to identify myeloid neoplasms.

METHODS:

Blood and bone marrow samples were evaluated for the fraction of CD177+ neutrophils by flow cytometry.

RESULTS:

Skewed high neutrophil CD177(%) was not associated with neoplasia, but skewed low neutrophil CD177(%) was highly correlated with clonal myeloid disorders at values less than 40%. Specificity of low neutrophil CD177(%) for clonal myeloid disorders was 87% with a 40% cutoff and 95% with a 30% cutoff. Findings were most pronounced for myelodysplasia, with 52% (11/21) containing fewer than 40% CD177+ neutrophils. Specificity was also suggested by normalization of neutrophil CD177(%) in four patients who reached morphologic remission after therapy for myelodysplasia or acute leukemia.

CONCLUSIONS:

Skewed low neutrophil CD177(%) is highly associated with clonal myeloid disorders, particularly myelodysplasia, and may be useful for detecting clonal myeloid disorders.

KEYWORDS:

CD177; Flow cytometry; Myelodysplasia; Neutrophil

PMID:
24124144
DOI:
10.1309/AJCPDFBEBQZW1OI7
[Indexed for MEDLINE]
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