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Anticancer Res. 2013 Oct;33(10):4319-24.

Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line.

Author information

1
Department of Digestive Surgery, Kawasaki Medical School, 577 Matsushima, Kurashiki, Okayama 710-0192, Japan. kubota@med.kawasaki-m.ac.jp.

Abstract

BACKGROUND/AIM:

The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1).

MATERIALS AND METHODS:

Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed.

RESULTS:

LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells.

CONCLUSION:

EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.

KEYWORDS:

Esophageal cancer; NF-κB; TE-1 cells; apoptosis; cytokines; eicosapentaenoic acid

PMID:
24122998
[Indexed for MEDLINE]
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