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Nat Methods. 2013 Dec;10(12):1206-8. doi: 10.1038/nmeth.2691. Epub 2013 Oct 13.

Detergent-free mass spectrometry of membrane protein complexes.

Author information

1
Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.

Abstract

We developed a method that allows release of intact membrane protein complexes from amphipols, bicelles and nanodiscs in the gas phase for observation by mass spectrometry (MS). Current methods involve release of membrane protein complexes from detergent micelles, which reveals subunit composition and lipid binding. We demonstrated that oligomeric complexes or proteins requiring defined lipid environments are stabilized to a greater extent in the absence of detergent.

PMID:
24122040
PMCID:
PMC3868940
DOI:
10.1038/nmeth.2691
[Indexed for MEDLINE]
Free PMC Article

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