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Anal Chim Acta. 2013 Oct 24;800:36-42. doi: 10.1016/j.aca.2013.09.014. Epub 2013 Sep 18.

Proteomic platform for the identification of proteins in olive (Olea europaea) pulp.

Author information

1
Dipartimento di Chimica, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy. Electronic address: annalaura.capriotti@uniroma1.it.

Abstract

The nutritional and cancer-protective properties of the oil extracted mechanically from the ripe fruits of Olea europaea trees are attracting constantly more attention worldwide. The preparation of high-quality protein samples from plant tissues for proteomic analysis poses many challenging problems. In this study we employed a proteomic platform based on two different extraction methods, SDS and CHAPS based protocols, followed by two precipitation protocols, TCA/acetone and MeOH precipitation, in order to increase the final number of identified proteins. The use of advanced MS techniques in combination with the Swissprot and NCBI Viridiplantae databases and TAIR10 Arabidopsis database allowed us to identify 1265 proteins, of which 22 belong to O. europaea. The application of this proteomic platform for protein extraction and identification will be useful also for other proteomic studies on recalcitrant plant/fruit tissues.

KEYWORDS:

3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate; CHAPS; CMC; DTT; FDR; GO; Gel-free approach; High resolution mass spectrometry; Olive pulp; PPO; Plant proteomics; Protein extraction; RP; RPK; RT; SDS; SPE; TCA; critical micelle concentration; dithiothreitol; false discovery rate; gene ontology; polyphenol oxidase; receptor like protein kinase; reversed-phase; room temperature; sodium dodecyl sulphate; solid phase extraction; trichloroacetic acid

PMID:
24120165
DOI:
10.1016/j.aca.2013.09.014
[Indexed for MEDLINE]

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