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J Neurochem. 2013 Nov;127(4):461-70. doi: 10.1111/jnc.12460. Epub 2013 Oct 21.

Genomic editing opens new avenues for zebrafish as a model for neurodegeneration.

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German Center for Neurodegenerative Diseases (DZNE), Munich, Germany; Munich Cluster for Systems Neurology (SyNergy), Munich, Germany.


Zebrafish has become a popular model organism to study human diseases. We will highlight the advantages and limitations of zebrafish as a model organism to study neurodegenerative diseases and introduce zinc finger nucleases, transcription activator-like effector nucleases, and the recently established clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeat-associated system for genome editing. The efficiency of the novel genome editing tools now greatly facilitates knock-out and, importantly, also makes knock-in approaches feasible in zebrafish. Genome editing in zebrafish avoids unspecific phenotypes caused by off-target effects and toxicity as frequently seen in conventional knock-down approaches.


CRISPR/Cas (clustered regularly interspaced short palindromic repeats); TALEN; genomics; neurodegeneration; zebrafish

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