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Org Lett. 2013 Nov 1;15(21):5528-30. doi: 10.1021/ol402585c. Epub 2013 Oct 11.

Efficient enzymatic synthesis of guanosine 5'-diphosphate-sugars and derivatives.

Author information

1
State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University , Tianjin 300071, China, Center for Diagnostics & Therapeutics and Department of Chemistry, Georgia State University , Atlanta, Georgia 30303, United States, and Department of Chemistry, University of California , Davis, One Shields Avenue, Davis, California 95616, United States.

Abstract

An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK_15697), a guanosine 5'-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.

PMID:
24117142
PMCID:
PMC3915774
DOI:
10.1021/ol402585c
[Indexed for MEDLINE]
Free PMC Article

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