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FEBS Lett. 2013 Nov 15;587(22):3722-8. doi: 10.1016/j.febslet.2013.09.042. Epub 2013 Oct 7.

Generation and characterization of a rabbit monoclonal antibody site-specific for tau O-GlcNAcylated at serine 400.

Author information

1
EMD Serono Research & Development Institute, Billerica, MA 01821, United States.

Abstract

Aggregation of tau into paired helical filaments is a pathological process leading to neurotoxicity in Alzheimer's disease and other tauopathies. Tau is posttranslationally modified by O-linked N-acetylglucosamine (O-GlcNAc), and increasing tau O-GlcNAcylation may protect against its aggregation. Research tools to study the relationship between tau aggregation and tau O-GlcNAcylation have not been widely available. Here we describe the generation of a rabbit monoclonal antibody specific for tau O-GlcNAcylated at Ser400 (O-tau(S400)). We show the utility of this antibody for in vitro and in vivo experiments to investigate the function of O-GlcNAc modifications of tau at Ser400.

KEYWORDS:

Monoclonal antibody; O-GlcNAc; OGA; Site-specific; Tau

PMID:
24113653
DOI:
10.1016/j.febslet.2013.09.042
[Indexed for MEDLINE]
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