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Methods Mol Biol. 2014;1076:29-42. doi: 10.1007/978-1-62703-649-8_2.

Steady-state fluorescence polarization/anisotropy for the study of protein interactions.

Author information

1
Department Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Honolulu, HI, USA.

Abstract

Fluorescence methods are often employed for the characterization of molecular interactions. In particular, polarization/anisotropy studies are widely utilized in the life sciences as they allow quantification of protein interactions in the micro- and nanomolar concentration range. Herein we shall briefly describe the theoretical aspects of polarization/anisotropy and outline an experiment for determination of the dissociation constant for a protein-ligand complex.

PMID:
24108621
DOI:
10.1007/978-1-62703-649-8_2
[Indexed for MEDLINE]

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