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Sci Rep. 2013 Oct 10;3:2847. doi: 10.1038/srep02847.

Live pigs produced from genome edited zygotes.

Author information

1
The Roslin Institute and R(D)SVS, Easter Bush Campus, University of Edinburgh, Edinburgh, EH25 9RG, UK.

Abstract

Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

PMID:
24108318
DOI:
10.1038/srep02847
[Indexed for MEDLINE]
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