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Pediatr Res. 2013 Dec;74(6):689-97. doi: 10.1038/pr.2013.163. Epub 2013 Sep 4.

Tracking donor RBC survival in premature infants: agreement of multiple populations of biotin-labeled RBCs with Kidd antigen-mismatched RBCs.

Author information

1
Department of Pediatrics, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa.
2
Department of Biochemistry & Molecular Biology and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
3
1] Department of Pediatrics, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa [2] Department of Pathology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa.
4
Department of Biostatistics, University of Iowa College of Public Health, Iowa City, Iowa.

Abstract

BACKGROUND:

Anemia, a common condition among critically ill premature infants, is affected by red blood cell (RBC) survival (RCS). We hypothesized that transfused allogeneic Kidd antigen-mismatched RBCs would demonstrate the same concurrent RCS tracking as RBCs multilabeled at separate, discrete low densities with biotin (BioRBCs).

METHODS:

Allogeneic RBCs from adult donors were labeled at four biotin densities, mixed, and transfused into 17 anemic premature infants. Nine of the donors and neonates were Kidd antigen mismatched. Serial posttransfusion blood samples were assayed for up to 8 wk by flow cytometry to track the survival of the proportions of Kidd antigen-mismatched and Kidd antigen-biotinylated RBCs.

RESULTS:

Using linear mixed modeling to compare results, RCS of the three lowest BioRBC densities was similar to RCS by Kidd antigen mismatch and to one another. RCS of RBCs labeled at the highest BioRBC density was shortened.

CONCLUSION:

RCS of different populations of RBCs can be tracked concurrently and reliably using the three lowest BioRBC densities. Although comparable RCS results can be achieved using Kidd antigen mismatches, BioRBCs are preferred for investigating neonatal anemia because biotin labeling of both allogeneic and autologous RBCs is possible.

PMID:
24108188
PMCID:
PMC3913052
DOI:
10.1038/pr.2013.163
[Indexed for MEDLINE]
Free PMC Article
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