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J Infect Dis. 2014 Jan 15;209(2):192-202. doi: 10.1093/infdis/jit450. Epub 2013 Oct 7.

Molecular characterization of a novel botulinum neurotoxin type H gene.

Author information

1
Infant Botulism Treatment and Prevention Program, California Department of Public Health, Richmond, California.

Abstract

We sequenced the 2 botulinum toxin gene clusters of Clostridium botulinum strain IBCA10-7060 type Bh. The sequence of bont/H differed substantially from the sequences of the 7 known bont genes for toxin types A-G. The 5' one-third terminus of bont/H that codes for the botulinum toxin light chain differed markedly from the light chain coding sequences of toxin types A-G. The 3' two-thirds terminus of bont/H that codes for the botulinum toxin heavy chain contained a novel Hn translocation domain coding sequence and a nonneutralizing type A-like Hc binding domain coding sequence. bont/H was part of an orfX toxin gene cluster that was located at a unique chromosomal site distant from those used by other botulinum toxin gene clusters. The bont/B sequence was similar to that of subtype bont/B2 and was located within its ha toxin gene cluster at the oppA/brnQ site. Our findings further establish that C. botulinum IBCA10-7060 produces novel BoNT/H.

KEYWORDS:

Clostridium botulinum; botulinum toxin; botulinum toxin type H; botulism

PMID:
24106295
DOI:
10.1093/infdis/jit450
[Indexed for MEDLINE]

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