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J Fluoresc. 2014 Mar;24(2):403-9. doi: 10.1007/s10895-013-1305-8. Epub 2013 Oct 8.

Microspectroscopic Study of Liposome-to-cell Interaction Revealed by Förster Resonance Energy Transfer.

Author information

1
Institute for Scintillation Materials, NAS of Ukraine, 60 Lenin ave, 61001, Kharkov, Ukraine. ephimova@isma.kharkov.ua.
2
Slavyansk State Pedagogical University, 19 Batyuka str, Slavyansk, 84116, Ukraine.
3
Institute for Scintillation Materials, NAS of Ukraine, 60 Lenin ave, 61001, Kharkov, Ukraine.
4
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, 03022, Ukraine.

Abstract

We report the Förster resonance energy transfer (FRET)-labeling of liposomal vesicles as an effective approach to study in dynamics the interaction of liposomes with living cells of different types (rat hepatocytes, rat bone marrow, mouse fibroblast-like cells and human breast cancer cells) and cell organelles (hepatocyte nuclei). The in vitro experiments were performed using fluorescent microspectroscopic technique. Two fluorescent dyes (DiO as the energy donor and DiI as an acceptor) were preloaded in lipid bilayers of phosphatidylcholine liposomes that ensures the necessary distance between the dyes for effective FRET. The change in time of the donor and acceptor relative fluorescence intensities was used to visualize and trace the liposome-to-cell interaction. We show that FRET-labeling of liposome vesicles allows one to reveal the differences in efficiency and dynamics of these interactions, which are associated with composition, fluidity, and metabolic activity of cell plasma membranes.

KEYWORDS:

Fluorescent dyes; Förster resonance energy transfer; Liposome; Living cells

PMID:
24101211
DOI:
10.1007/s10895-013-1305-8
[Indexed for MEDLINE]

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