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Chem Commun (Camb). 2013 Nov 21;49(90):10605-7. doi: 10.1039/c3cc45459b.

Colorimetric detection of human papilloma virus by double isothermal amplification.

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  • 1Istituto Italiano di Tecnologia (IIT), Center for Bio-Molecular Nanotechnologies@UniLe, Via Barsanti - 73010 Arnesano, Lecce, Italy. pierpaolo.pompa@iit.it.

Abstract

We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.

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