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PLoS One. 2013 Oct 2;8(10):e76258. doi: 10.1371/journal.pone.0076258. eCollection 2013.

In vivo T cell activation induces the formation of CD209(+) PDL-2(+) dendritic cells.

Author information

1
Department of Pathology, Stanford University School of Medicine (Blood Center), Palo Alto, California, United States of America.

Abstract

Two critical functions of dendritic cells (DC) are to activate and functionally polarize T cells. Activated T cells can, in turn, influence DC maturation, although their effect on de novo DC development is poorly understood. Here we report that activation of T cells in mice, with either an anti-CD3 antibody or super antigen, drives the rapid formation of CD209(+)CD11b(+)CD11c(+) MHC II(+) DC from monocytic precursors (Mo-DC). GM-CSF is produced by T cells following activation, but surprisingly, it is not required for the formation of CD209(+) Mo-DC. CD40L, however, is critical for the full induction of Mo-DC following T cell activation. T cell induced CD209(+) Mo-DC are comparable to conventional CD209(-) DC in their ability to stimulate T cell proliferation. However, in contrast to conventional CD209(-) DC, CD209(+) Mo-DC fail to effectively polarize T cells, as indicated by a paucity of T cell cytokine production. The inability of CD209(+) Mo-DC to polarize T cells is partly explained by increased expression of PDL-2, since blockade of this molecule restores some polarizing capacity to the Mo-DC. These findings expand the range of signals capable of driving Mo-DC differentiation in vivo beyond exogenous microbial factors to include endogenous factors produced following T cell activation.

PMID:
24098455
PMCID:
PMC3788745
DOI:
10.1371/journal.pone.0076258
[Indexed for MEDLINE]
Free PMC Article

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