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Nanomedicine. 2014 Feb;10(2):483-90. doi: 10.1016/j.nano.2013.09.003. Epub 2013 Oct 2.

Anti-leishmanial activity of the antimicrobial peptide DRS 01 observed in Leishmania infantum (syn. Leishmania chagasi) cells.

Author information

1
REQUIMTE/Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, Porto, Portugal. Electronic address: peter.eaton@fc.up.pt.
2
Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus de Parnaíba, Universidade Federal do Piauí, UFPI, Parnaíba, PI, Brazil.
3
Laboratório de Pesquisas em Leishmanioses, Instituto de Doenças Tropicais Natan Portella, IDTNP, Teresina, Brazil.
4
REQUIMTE/Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, Porto, Portugal.

Abstract

Leishmaniasis is one of the most serious diseases in the world and can be lethal if untreated. This is especially the case for visceral leishmaniasis, which is commonly caused by Leishmania (L.) infantum and for which available medication is still inadequate. A recently described antimicrobial peptide DRS 01 has been reported to kill L. infantum promastigotes, but nothing is known about its mode of action or effect on the cell. In this paper we report the visualization of the interaction between DRS 01 and L. infantum promastigotes using two high resolution microscopic techniques: atomic force microscopy and scanning electron microscopy. The results show considerable morphological changes at and above the IC50 in the treated cells. Both membrane damage and flagella alterations were observed. The results strongly suggest a membrane-directed action for DRS 01 on the Leishmania species studied.

FROM THE CLINICAL EDITOR:

In this paper, the effects of DRS 01, an antimicrobial peptide, is studied in Leishmania infantum using atomic force microscopy as well as standard scanning electron microscopy techniques, with the conclusion of a membrane-based effect by DRS 01 on the parasites.

KEYWORDS:

Atomic force microscopy; Dermaseptins; Leishmania chagasi; Leishmania infantum; Scanning electron microscopy

PMID:
24096030
DOI:
10.1016/j.nano.2013.09.003
[Indexed for MEDLINE]

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