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PLoS One. 2013 Sep 27;8(9):e76475. doi: 10.1371/journal.pone.0076475. eCollection 2013.

Field-portable pixel super-resolution colour microscope.

Author information

1
Electrical Engineering Department, University of California Los Angeles, Los Angeles, California, United States of America.

Abstract

Based on partially-coherent digital in-line holography, we report a field-portable microscope that can render lensfree colour images over a wide field-of-view of e.g., >20 mm(2). This computational holographic microscope weighs less than 145 grams with dimensions smaller than 17×6×5 cm, making it especially suitable for field settings and point-of-care use. In this lensfree imaging design, we merged a colorization algorithm with a source shifting based multi-height pixel super-resolution technique to mitigate 'rainbow' like colour artefacts that are typical in holographic imaging. This image processing scheme is based on transforming the colour components of an RGB image into YUV colour space, which separates colour information from brightness component of an image. The resolution of our super-resolution colour microscope was characterized using a USAF test chart to confirm sub-micron spatial resolution, even for reconstructions that employ multi-height phase recovery to handle dense and connected objects. To further demonstrate the performance of this colour microscope Papanicolaou (Pap) smears were also successfully imaged. This field-portable and wide-field computational colour microscope could be useful for tele-medicine applications in resource poor settings.

PMID:
24086742
PMCID:
PMC3785454
DOI:
10.1371/journal.pone.0076475
[Indexed for MEDLINE]
Free PMC Article

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