Format

Send to

Choose Destination
See comment in PubMed Commons below
PLoS One. 2013 Sep 25;8(9):e75501. doi: 10.1371/journal.pone.0075501. eCollection 2013.

Bions: a family of biomimetic mineralo-organic complexes derived from biological fluids.

Author information

1
Laboratory of Nanomaterials, Chang Gung University, Gueishan, Taoyuan, Taiwan, Republic of China ; Center for Molecular and Clinical Immunology, Chang Gung University, Gueishan, Taoyuan, Taiwan, Republic of China ; Research Center of Bacterial Pathogenesis, Chang Gung University, Gueishan, Taoyuan, Taiwan, Republic of China.

Erratum in

  • PLoS One. 2014;9(3):e91496.

Abstract

Mineralo-organic nanoparticles form spontaneously in human body fluids when the concentrations of calcium and phosphate ions exceed saturation. We have shown previously that these mineralo-organic nanoparticles possess biomimetic properties and can reproduce the whole phenomenology of the so-called nanobacteria-mineralized entities initially described as the smallest microorganisms on earth. Here, we examine the possibility that various charged elements and ions may form mineral nanoparticles with similar properties in biological fluids. Remarkably, all the elements tested, including sodium, magnesium, aluminum, calcium, manganese, iron, cobalt, nickel, copper, zinc, strontium, and barium form mineralo-organic particles with bacteria-like morphologies and other complex shapes following precipitation with phosphate in body fluids. Upon formation, these mineralo-organic particles, which we term bions, invariably accumulate carbonate apatite during incubation in biological fluids; yet, the particles also incorporate additional elements and thus reflect the ionic milieu in which they form. Bions initially harbor an amorphous mineral phase that gradually converts to crystals in culture. Our results show that serum produces a dual inhibition-seeding effect on bion formation. Using a comprehensive proteomic analysis, we identify a wide range of proteins that bind to these mineral particles during incubation in medium containing serum. The two main binding proteins identified, albumin and fetuin-A, act as both inhibitors and seeders of bions in culture. Notably, bions possess several biomimetic properties, including the possibility to increase in size and number and to be sub-cultured in fresh culture medium. Based on these results, we propose that bions represent biological, mineralo-organic particles that may form in the body under both physiological and pathological homeostasis conditions. These mineralo-organic particles may be part of a physiological cycle that regulates the function, transport and disposal of elements and minerals in the human body.

PMID:
24086546
PMCID:
PMC3783384
DOI:
10.1371/journal.pone.0075501
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Public Library of Science Icon for PubMed Central
    Loading ...
    Support Center