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PLoS Comput Biol. 2013;9(9):e1003245. doi: 10.1371/journal.pcbi.1003245. Epub 2013 Sep 26.

Barcoding T cell calcium response diversity with methods for automated and accurate analysis of cell signals (MAAACS).

Author information

1
Centre d'Immunologie de Marseille-Luminy (CIML), Aix-Marseille University, UM2, Marseille, France ; Institut National de la Santé et de la Recherche Médicale (Inserm), U1104, Marseille, France ; Centre National de la Recherche Scientifique (CNRS) UMR7280, Marseille, France.

Abstract

We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy. Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals (MAAACS), a fully customized program that associates a high throughput tracking algorithm, an intuitive reconnection routine and a statistical platform to provide, at a glance, the calcium barcode of a population of individual T-cells. Combined with a sensitive calcium probe, this method allowed us to unravel the heterogeneity in shape and intensity of the calcium response in T cell populations and especially in naive T cells, which display intracellular calcium oscillations upon stimulation by antigen presenting cells.

PMID:
24086124
PMCID:
PMC3784497
DOI:
10.1371/journal.pcbi.1003245
[Indexed for MEDLINE]
Free PMC Article

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