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J Chromatogr A. 2013 Nov 8;1315:28-35. doi: 10.1016/j.chroma.2013.09.046. Epub 2013 Sep 18.

Construction of graphene oxide magnetic nanocomposites-based on-chip enzymatic microreactor for ultrasensitive pesticide detection.

Author information

1
Department of Chemistry, Nanchang University, Nanchang 330031, China.

Abstract

A new strategy for facile construction of graphene oxide magnetic nanocomposites (GO/Fe3O4 MNCs)-based on-chip enzymatic microreactor and ultrasensitive pesticide detection has been proposed. GO/Fe3O4 MNCs were first prepared through an in situ chemical deposition strategy. Then, acetylcholinesterase (AChE) was adsorbed onto the GO/Fe3O4 surface to form GO/Fe3O4/AChE MNCs which was locally packed into PDMS microchannel simply with the help of external magnetic field to form an on-chip enzymatic microreactor. The constructed GO/Fe3O4/AChE MNCs-based enzymatic microreactor not only have the magnetism of Fe3O4 NPs that make them conveniently manipulated by an external magnetic field, but also have the larger surface and excellent biocompatibility of graphene which can incorporate much more AChE molecules and well maintain their biological activity. On the basis of the AChE inhibition principle, a novel on-chip enzymatic microreactor was constructed for analyzing dimethoate which is usually used as a model of organophosphorus pesticides. Under optimal conditions, a linear relationship between the inhibition rates of AChE and the concentration of dimethoate from 1 to 20 μgL(-1) with a detection limit of 0.18 μgL(-1) (S/N=3) was obtained. The developed electrophoretic and magnetic-based chip exhibited excellent reproducibility and stability with no decrease in the activity of enzyme for more than 20 repeated measurements over one week period, which provided a new and promising tool for the analysis of enzyme inhibitors with low cost and excellent performance.

KEYWORDS:

Acetylcholinesterase; Graphene oxide-magnetic nanocomposites; On-chip microreactor; Organophosphorus pesticides; PDMS

PMID:
24084001
DOI:
10.1016/j.chroma.2013.09.046
[Indexed for MEDLINE]

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