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Nat Methods. 2013 Oct;10(10):957-63. doi: 10.1038/nmeth.2649.

Cas9 as a versatile tool for engineering biology.

Author information

1
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

Abstract

RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the genomes of diverse organisms. We believe tools and techniques based on Cas9, a single unifying factor capable of colocalizing RNA, DNA and protein, will grant unprecedented control over cellular organization, regulation and behavior. Here we describe the Cas9 targeting methodology, detail current and prospective engineering advances and suggest potential applications ranging from basic science to the clinic.

PMID:
24076990
PMCID:
PMC4051438
DOI:
10.1038/nmeth.2649
[Indexed for MEDLINE]
Free PMC Article

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