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Genomics Proteomics Bioinformatics. 2013 Oct;11(5):312-9. doi: 10.1016/j.gpb.2013.09.003. Epub 2013 Sep 27.

Induced neural stem cells generated from rat fibroblasts.

Author information

1
Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Department of Cell and Neurobiology, University of Southern California, Los Angeles, CA 90033, USA; Department of Neurology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi 214023, China.

Abstract

The generation of induced tissue-specific stem cells has been hampered by the lack of well-established methods for the maintenance of pure tissue-specific stem cells like the ones we have for embryonic stem (ES) cell cultures. Using a cocktail of cytokines and small molecules, we demonstrate that primitive neural stem (NS) cells derived from mouse ES cells and rat embryos can be maintained. Furthermore, using the same set of cytokines and small molecules, we show that induced NS (iNS) cells can be generated from rat fibroblasts by forced expression of the transcriptional factors Oct4, Sox2 and c-Myc. The generation and long-term maintenance of iNS cells could have wide and momentous implications.

KEYWORDS:

Fibroblasts; Induced neural stem cells; Induced pluripotent stem cells; Rat; Stem cell self-renewal

PMID:
24076032
PMCID:
PMC3908478
DOI:
10.1016/j.gpb.2013.09.003
[Indexed for MEDLINE]
Free PMC Article
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