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PLoS Genet. 2013;9(9):e1003784. doi: 10.1371/journal.pgen.1003784. Epub 2013 Sep 19.

MEIOB targets single-strand DNA and is necessary for meiotic recombination.

Author information

1
Univ. Paris Diderot, Sorbonne Paris Cité, Laboratory of Development of the Gonads, Unit of Stem Cells and Radiation, UMR 967, Fontenay aux Roses, France ; CEA, DSV, iRCM, SCSR, LDG, Fontenay aux Roses, France ; INSERM, Unité 967, Fontenay aux Roses, France ; Univ. Paris-Sud, UMR 967, Fontenay aux Roses, France.

Abstract

Meiotic recombination is a mandatory process for sexual reproduction. We identified a protein specifically implicated in meiotic homologous recombination that we named: meiosis specific with OB domain (MEIOB). This protein is conserved among metazoan species and contains single-strand DNA binding sites similar to those of RPA1. Our studies in vitro revealed that both recombinant and endogenous MEIOB can be retained on single-strand DNA. Those in vivo demonstrated the specific expression of Meiob in early meiotic germ cells and the co-localization of MEIOB protein with RPA on chromosome axes. MEIOB localization in Dmc1 (-/-) spermatocytes indicated that it accumulates on resected DNA. Homologous Meiob deletion in mice caused infertility in both sexes, due to a meiotic arrest at a zygotene/pachytene-like stage. DNA double strand break repair and homologous chromosome synapsis were impaired in Meiob (-/-) meiocytes. Interestingly MEIOB appeared to be dispensable for the initial loading of recombinases but was required to maintain a proper number of RAD51 and DMC1 foci beyond the zygotene stage. In light of these findings, we propose that RPA and this new single-strand DNA binding protein MEIOB, are essential to ensure the proper stabilization of recombinases which is required for successful homology search and meiotic recombination.

PMID:
24068956
PMCID:
PMC3778009
DOI:
10.1371/journal.pgen.1003784
[Indexed for MEDLINE]
Free PMC Article

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