Format

Send to

Choose Destination
Dev Biol. 2013 Nov 15;383(2):321-30. doi: 10.1016/j.ydbio.2013.09.021. Epub 2013 Sep 21.

The function of Notch signalling in segment formation in the crustacean Daphnia magna (Branchiopoda).

Author information

1
School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK; Department of Neurobiology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.

Abstract

Ten years ago we showed for the first time that Notch signalling is required in segmentation in spiders, indicating the existence of similar mechanisms in arthropod and vertebrate segmentation. However, conflicting results in various arthropod groups hampered our understanding of the ancestral function of Notch in arthropod segmentation. Here we fill a crucial data gap in arthropods and analyse segmentation in a crustacean embryo. We analyse the expression of homologues of the Drosophila and vertebrate segmentation genes and show that members of the Notch signalling pathway are expressed at the same time as the pair-rule genes. Furthermore, inactivation of Notch signalling results in irregular boundaries of the odd-skipped-like expression domains and affects the formation of segments. In severe cases embryos appear unsegmented. We suggest two scenarios for the function of Notch signalling in segmentation. The first scenario agrees with a segmentation clock involving Notch signalling, while the second scenario discusses an alternative mechanism of Notch function which is integrated into a hierarchical segmentation cascade.

KEYWORDS:

Delta-Notch signalling; Hes; Pair-rule genes; Segmentation cascade; odd-skipped; snail

PMID:
24063806
DOI:
10.1016/j.ydbio.2013.09.021
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center