Send to

Choose Destination
See comment in PubMed Commons below
Circ Res. 2013 Dec 6;113(12):1308-19. doi: 10.1161/CIRCRESAHA.113.301867. Epub 2013 Sep 23.

Cyclophilin D modulates mitochondrial acetylome.

Author information

  • 1From the Systems Biology Center (T.T.M.N., S.M., J.S., E.M.), Division of Cardiovascular Sciences (R.W.), Proteomics Core Facility (Y.C., G.W., M.G.), and Center for Molecular Medicine (M.N.S.), National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD; and Department of Pathology, Johns Hopkins Medical Center, Baltimore, MD (C.S.).



Mice lacking cyclophilin D (CypD(-/-)), a mitochondrial chaperone protein, have altered cardiac metabolism. As acetylation has been shown to regulate metabolism, we tested whether changes in protein acetylation might play a role in these metabolic changes in CypD(-/-) hearts.


Our aim was to test the hypothesis that loss of CypD alters the cardiac mitochondrial acetylome.


To identify changes in lysine-acetylated proteins and to map acetylation sites after ablation of CypD, we subjected tryptic digests of isolated cardiac mitochondria from wild-type and CypD(-/-) mice to immunoprecipitation using agarose beads coupled to antiacetyl lysine antibodies followed by mass spectrometry. We used label-free analysis for the relative quantification of the 875 common peptides that were acetylated in wild-type and CypD(-/-) samples and found 11 peptides (10 proteins) decreased and 96 peptides (48 proteins) increased in CypD(-/-) samples. We found increased acetylation of proteins in fatty acid oxidation and branched-chain amino acid metabolism. To evaluate whether this increase in acetylation might play a role in the inhibition of fatty acid oxidation that was previously reported in CypD(-/-) hearts, we measured the activity of l-3-hydroxyacyl-CoA dehydrogenase, which was acetylated in the CypD(-/-) hearts. Consistent with the hypothesis, l-3-hydroxyacyl-CoA dehydrogenase activity was inhibited by ≈50% compared with the wild-type mitochondria.


These results implicate a role for CypD in modulating protein acetylation. Taken together, these results suggest that ablation of CypD leads to changes in the mitochondrial acetylome, which may contribute to altered mitochondrial metabolism in CypD(-/-) mice.


acetylation; cyclophilin D; mitochondria; proteomics; sirtuin 3

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Support Center