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Nat Methods. 2013 Nov;10(11):1096-8. doi: 10.1038/nmeth.2639. Epub 2013 Sep 22.

Smart-seq2 for sensitive full-length transcriptome profiling in single cells.

Author information

1
Ludwig Institute for Cancer Research, Stockholm, Sweden.

Abstract

Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage, the sensitivity or the throughput. Here, we introduce Smart-seq2 with improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells. Smart-seq2 transcriptome libraries have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.

PMID:
24056875
DOI:
10.1038/nmeth.2639
[Indexed for MEDLINE]

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