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Nat Methods. 2013 Nov;10(11):1105-7. doi: 10.1038/nmeth.2647. Epub 2013 Sep 22.

Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.

Author information

1
1] Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, Maryland, USA. [2] Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. [3] Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. [4].

Abstract

Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range.

PMID:
24056873
PMCID:
PMC3860264
DOI:
10.1038/nmeth.2647
[Indexed for MEDLINE]
Free PMC Article

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