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Carbohydr Res. 2013 Nov 15;381:6-11. doi: 10.1016/j.carres.2013.08.013. Epub 2013 Aug 24.

Revised structures for the predominant O-polysaccharides expressed by Burkholderia pseudomallei and Burkholderia mallei.

Author information

1
Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA. Electronic address: cheiss@uga.edu.

Abstract

O-Polysaccharides (OPS) were isolated from purified Burkholderia pseudomallei and Burkholderia mallei lipopolysaccharides by mild-acid hydrolysis and gel-permeation chromatography. 1-D and 2-D (1)H and (13)C NMR spectroscopy experiments revealed that the OPS antigens were unbranched heteropolymers with the following structures: Collectively, our results demonstrate that the predominant OPS antigens expressed by B. pseudomallei and B. mallei isolates are structurally more complex than previously described and provide evidence that different capping residues are used by these closely related pathogens to terminate chain elongation. Additionally, they confirm that Burkholderia thailandensis and B. pseudomallei express OPS antigens that are essentially identical to one another.

KEYWORDS:

Burkholderia mallei; Burkholderia pseudomallei; Burkholderia thailandensis; Lipopolysaccharide; O-polysaccharide; Structure

PMID:
24056008
PMCID:
PMC3923507
DOI:
10.1016/j.carres.2013.08.013
[Indexed for MEDLINE]
Free PMC Article

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