Characterization of cellulose acetates according to DS and molar mass using two-dimensional chromatography

Hewa Othman Ghareeb; Wolfgang Radke

doi:10.1016/j.carbpol.2013.07.061

Characterization of cellulose acetates according to DS and molar mass using two-dimensional chromatography

Carbohydr Polym. 2013 Nov 6;98(2):1430-7. doi: 10.1016/j.carbpol.2013.07.061. Epub 2013 Aug 6.

Authors

Hewa Othman Ghareeb¹, Wolfgang Radke

Affiliation

¹ Fraunhofer-Institut für Betriebsfestigkeit und Systemzuverlässigkeit LBF, Bereich Kunststoffe, Schlossgartenstraße 6, D-64289 Darmstadt, Germany.

PMID: 24053824
DOI: 10.1016/j.carbpol.2013.07.061

Abstract

A two-dimensional liquid chromatographic method (2D LC) was developed to analyze the heterogeneities of cellulose acetates (CA) in the DS-range DS=1.5-2.9 with respect to both, molar mass and degree of substitution (DS). The method uses gradient liquid chromatography (HPLC) as the first dimension in order to separate by DS followed by separation of the different fractions by size (SEC) in the second dimension. The 2D experiments revealed different correlations between gradient and SEC elution volume. These correlations might arise from differences in the synthetic conditions. The newly developed 2D LC separation therefore provides new insights into the heterogeneity of CAs.

Keywords: Cellulose acetate; Degree of substitution; Gradient high performance liquid chromatography; Molar mass; Size exclusion chromatography; Two-dimensional liquid chromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cellulose / analogs & derivatives*
Cellulose / isolation & purification
Chromatography, Gel
Chromatography, Liquid / methods*
Factor Analysis, Statistical
Molecular Weight

Substances

acetylcellulose
Cellulose