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ACS Nano. 2013 Oct 22;7(10):8605-15. doi: 10.1021/nn403311c. Epub 2013 Sep 26.

Mechanisms of nanoparticle-mediated siRNA transfection by melittin-derived peptides.

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1
Computational and Molecular Biophysics, Washington University School of Medicine , St. Louis, Missouri. 63108, United States.

Abstract

Traditional peptide-mediated siRNA transfection via peptide transduction domains exhibits limited cytoplasmic delivery of siRNA due to endosomal entrapment. This work overcomes these limitations with the use of membrane-destabilizing peptides derived from melittin for the knockdown of NFkB signaling in a model of adult T-cell leukemia/lymphoma. While the mechanism of siRNA delivery into the cytoplasmic compartment by peptide transduction domains has not been well studied, our analysis of melittin derivatives indicates that concurrent nanocomplex disassembly and peptide-mediated endosomolysis are crucial to siRNA transfection. Importantly, in the case of the most active derivative, p5RHH, this process is initiated by acidic pH, indicating that endosomal acidification after macropinocytosis can trigger siRNA release into the cytoplasm. These data provide general principles regarding nanocomplex response to endocytosis, which may guide the development of peptide/siRNA nanocomplex-based transfection.

PMID:
24053333
PMCID:
PMC4013830
DOI:
10.1021/nn403311c
[Indexed for MEDLINE]
Free PMC Article

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