Format

Send to

Choose Destination
Stem Cell Reports. 2013 Jun 4;1(1):46-52. doi: 10.1016/j.stemcr.2013.03.003. eCollection 2013.

WNT3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hESCs.

Author information

1
Howard Hughes Medical Institute, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA ; Program in Cellular and Molecular Medicine, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA ; Division of Hematology/Oncology, Department of Pediatrics, Boston Children's Hospital, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.

Abstract

Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of different human embryonic stem cell (hESC)/induced PSC (iPSC) lines makes the selection of an appropriate cell line for the differentiation of a particular cell lineage difficult. Here, we report identification of WNT3 as a biomarker capable of predicting definitive endoderm (DE) differentiation potential of hESCs. We show that the mRNA level of WNT3 in hESCs correlates with their DE differentiation efficiency. In addition, manipulations of hESCs through WNT3 knockdown or overexpression can respectively inhibit or promote DE differentiation in a WNT3 level-dependent manner. Finally, analysis of several hESC lines based on their WNT3 expression levels allowed accurate prediction of their DE differentiation potential. Collectively, our study supports the notion that WNT3 can serve as a biomarker for predicting DE differentiation potential of hESCs.

PMID:
24052941
PMCID:
PMC3757741
DOI:
10.1016/j.stemcr.2013.03.003
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center