Spectrophotometric determination of hydrogen peroxide with osmium(VIII) and m-carboxyphenylfluorone

Mitsuru Hoshino; Shinichiro Kamino; Mitsunobu Doi; Shingo Takada; Shota Mitani; Rika Yanagihara; Mamiko Asano; Takako Yamaguchi; Yoshikazu Fujita

doi:10.1016/j.saa.2013.08.048

Spectrophotometric determination of hydrogen peroxide with osmium(VIII) and m-carboxyphenylfluorone

Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jan 3:117:814-6. doi: 10.1016/j.saa.2013.08.048. Epub 2013 Aug 25.

Authors

Mitsuru Hoshino¹, Shinichiro Kamino, Mitsunobu Doi, Shingo Takada, Shota Mitani, Rika Yanagihara, Mamiko Asano, Takako Yamaguchi, Yoshikazu Fujita

Affiliation

¹ Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki, Osaka 569-1094, Japan. Electronic address: Mitsuru.Hoshino@gmail.com.

PMID: 24050957
DOI: 10.1016/j.saa.2013.08.048

Abstract

Spectrophotometric determination of hydrogen peroxide was accomplished with osmium(VIII) and m-carboxyphenylfluorone (MCPF) in the presence of cetyltrimethylammonium chloride (CTAC). In the determination of hydrogen peroxide based on the fading of the color of osmium(VIII)-MCPF complex, Beer's law was obeyed in the range 20-406 ng mL(-1), with an effective molar absorption coefficient (at 580 nm) of 5.21×10(4) L mol(-1) cm(-1) and a relative standard deviation of 0.33% (n=6). Further, we performed the characterization of MCPF and obtained the crystal structure.

Keywords: Colored complex; Hydrogen peroxide; Osmium(VIII); Spectrophotometry; m-Carboxyphenylfluorone.

MeSH terms

Cetrimonium
Cetrimonium Compounds
Crystallization
Crystallography, X-Ray
Fluoresceins / chemistry*
Hydrogen Peroxide / analysis*
Models, Chemical
Osmium / chemistry*
Spectrophotometry / methods*

Substances

4-carboxyphenylfluorone
Cetrimonium Compounds
Fluoresceins
Osmium
Hydrogen Peroxide
Cetrimonium