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Neuron. 2013 Sep 18;79(6):1067-77. doi: 10.1016/j.neuron.2013.07.012.

Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels.

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  • 1Department of Medicine, Imperial College London, London, W12 0NN, UK; School of Engineering and Materials Science, Queen Mary, University of London, London E1 4NS, UK. Electronic address: p.novak@qmul.ac.uk.

Abstract

Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and ion-channel complement of far more abundant small synaptic terminals (≤ 1 μm) remain poorly understood. Here we report a method based on superresolution scanning ion conductance imaging of small synapses in culture at approximately 100-150 nm 3D resolution, which allows presynaptic patch-clamp recordings in all four configurations (cell-attached, inside-out, outside-out, and whole-cell). Using this technique, we report presynaptic recordings of K(+), Na(+), Cl(-), and Ca(2+) channels. This semiautomated approach allows direct investigation of the distribution and properties of presynaptic ion channels at small central synapses.

PMID:
24050398
PMCID:
PMC3781326
DOI:
10.1016/j.neuron.2013.07.012
[PubMed - indexed for MEDLINE]
Free PMC Article

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