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ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20.

One-step cloning and chromosomal integration of DNA.

Author information

1
Department of Bioengineering and ‡Department of Pediatrics, Stanford University , California 94305, United States.

Abstract

We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.

PMID:
24050148
DOI:
10.1021/sb400021j
[Indexed for MEDLINE]

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