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J Hazard Mater. 2013 Nov 15;262:297-303. doi: 10.1016/j.jhazmat.2013.08.051. Epub 2013 Aug 27.

Mechanism of uranium(VI) uptake by Saccharomyces cerevisiae under environmentally relevant conditions: batch, HRTEM, and FTIR studies.

Author information

1
School of Nuclear Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730000, PR China. Electronic address: lux2009@lzu.edu.cn.

Abstract

Biosorption is of significance for the safety evaluation of high-level nuclear wastes repositories and remediation of radioactive contamination places. Quantitive study and structural characterization of uranium uptake by both live and heat-killed Saccharomyces cerevisiae at environmentally relevant uranium concentration and with different ionic strengths were carried out. Kinetic investigation showed the equilibrium reached within 15 min. In equilibrium studies, pH shift towards neutral indicated release of hydroxyl ions. pH was the most important factor, which partly affected electrostatic interaction between uranyl ions and S. cerevisiae surface. The high ionic strength inhibited biosorption capacity, which can be explained by a competitive reaction between sodium ions and uranyl ions. Heat killing process significantly enhanced biosorption capacity, showing an order of magnitude higher than that of live cells. High resolution transmission electron microscopy (HRTEM) coupled with energy dispersive X-ray (EDX) showed needle-like uranium-phosphate precipitation formed on the cell walls for both live and heat-killed cells. Besides, dark-field micrographs displayed considerable similar uranium-phosphate precipitation presented outside the heat-killed cells. The phosphate released during heat-killing process. FTIR illustrated function groups hydroxyl, carboxyl, phosphate, and amino groups played important role in complexation with uranium.

KEYWORDS:

Bioremediation; FTIR; HRTEM; Saccharomyces cerevisiae; Uranium(VI)

PMID:
24041822
DOI:
10.1016/j.jhazmat.2013.08.051
[Indexed for MEDLINE]
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