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PLoS One. 2013 Sep 11;8(9):e73833. doi: 10.1371/journal.pone.0073833. eCollection 2013.

Previous exposure to an RNA virus does not protect against subsequent infection in Drosophila melanogaster.

Author information

1
Department of Genetics, University of Cambridge, Cambridge, United Kingdom.

Abstract

BACKGROUND:

Immune priming has been shown to occur in a wide array of invertebrate taxa, with individuals exposed to a pathogen showing increased protection upon subsequent exposure. However, the mechanisms underlying immune priming are poorly understood. The antiviral RNAi response in Drosophila melanogaster is an ideal candidate for providing a specific and acquired response to subsequent infection. We exposed D. melanogaster to two challenges of a virus known to produce an antiviral RNAi response, to examine whether any protective effects of prior exposure on survival were observed.

RESULTS:

In this experiment we found no evidence that prior exposure to Drosophila C Virus (DCV) protects flies from a subsequent lethal challenge, with almost identical levels of mortality in flies previously exposed to DCV or a control.

CONCLUSIONS:

Our results confirm the finding that 'acquired' immune responses are not ubiquitous across all invertebrate-pathogen interactions. We discuss why we may have observed no effect in this study, with focus on the mechanistic basis of the RNAi pathway.

PMID:
24040086
PMCID:
PMC3770682
DOI:
10.1371/journal.pone.0073833
[Indexed for MEDLINE]
Free PMC Article

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