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PLoS Pathog. 2013;9(9):e1003619. doi: 10.1371/journal.ppat.1003619. Epub 2013 Sep 5.

Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish.

Author information

1
Institut Pasteur, Macrophages et Développement de l'Immunité, Department of Developmental and Stem Cells Biology, Paris, France ; CNRS URA2578, Paris, France ; Université Pierre et Marie Curie, Paris, France.

Abstract

Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of ∼14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.

PMID:
24039582
PMCID:
PMC3764224
DOI:
10.1371/journal.ppat.1003619
[Indexed for MEDLINE]
Free PMC Article

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