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Angew Chem Int Ed Engl. 2013 Oct 25;52(44):11534-7. doi: 10.1002/anie.201305875. Epub 2013 Sep 13.

Rapid high-specificity microRNA detection using a two-stage isotachophoresis assay.

Author information

1
Department of Mechanical Engineering, Stanford University, 440 Escondido Mall, Building 530, Room 225, Stanford, CA 94305 (USA).

Abstract

Focusing in on the small: A two-stage microRNA detection assay uses electrokinetic focusing to speed up hybridization and a functionalized hydrogel for affinity purification. This method detects microRNAs in 15 minutes with single-nucleotide specificity, and processes only 5 ng of total RNA.

KEYWORDS:

RNA recognition; RNA structures; analytical methods; hybridization assay; isotachophoresis

PMID:
24038732
DOI:
10.1002/anie.201305875
[Indexed for MEDLINE]

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