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J Immunol Methods. 2013 Nov 29;397(1-2):71-6. doi: 10.1016/j.jim.2013.09.001. Epub 2013 Sep 11.

A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions.

Author information

1
Thayer School of Engineering, Dartmouth College, Hanover, NH, USA.

Abstract

Mucosal tissues represent the front line in defense against potential pathogens, and one means by which mucosa provide protection is via the secretion of antimicrobials which can interfere with potential pathogens as well as recruit and modify the responses of immune cells. Here we describe adaptation of ELISA assays to microsphere format, facilitating simultaneous quantification of antimicrobial peptides including elafin, MIP3α, HBD2, HBD3, SLPI, RANTES, SDF1, lactoferrin, LL-37, and HNP1-3. The multiplexed assay exhibits excellent reproducibility, shows linearity over a two order of magnitude concentration range for most analytes, is compatible with biological fluids such as cervicovaginal lavage fluid, and presents significant cost and sample savings relative to traditional ELISA assays.

KEYWORDS:

Antimicrobials; Chemokines; Cytokines; Female reproductive tract; Mucosal secretions; Multiplex assay

PMID:
24035708
PMCID:
PMC3819932
DOI:
10.1016/j.jim.2013.09.001
[Indexed for MEDLINE]
Free PMC Article
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