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Diagn Microbiol Infect Dis. 2013 Nov;77(3):270-2. doi: 10.1016/j.diagmicrobio.2013.06.006. Epub 2013 Sep 10.

Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus.

Author information

1
Multidrug-resistant organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, MD, USA. Electronic address: patrick.t.mcgann4@mail.mil.

Abstract

We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA submitted to a surveillance network.

KEYWORDS:

Chlorhexidine; MRSA; MupA; Mupirocin; QacA

[Indexed for MEDLINE]

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