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Methods Enzymol. 2013;530:183-92. doi: 10.1016/B978-0-12-420037-1.00010-5.

Polysome analysis of mammalian cells.

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1
Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Abstract

To assess the global translational level of mammalian cells (see similar protocols for bacteria and yeast on Analysis of polysomes from bacteria, Polysome Profile Analysis - Yeast and Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).

KEYWORDS:

Cell lysate; Genome-wide analysis techniques; Immobilize ribosomes; Mammalian cells; Polysome analysis; Size fractionation; Sucrose density gradient centrifugation

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