Send to

Choose Destination
See comment in PubMed Commons below
Methods Enzymol. 2013;530:183-92. doi: 10.1016/B978-0-12-420037-1.00010-5.

Polysome analysis of mammalian cells.

Author information

Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.


To assess the global translational level of mammalian cells (see similar protocols for bacteria and yeast on Analysis of polysomes from bacteria, Polysome Profile Analysis - Yeast and Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).


Cell lysate; Genome-wide analysis techniques; Immobilize ribosomes; Mammalian cells; Polysome analysis; Size fractionation; Sucrose density gradient centrifugation

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center